Manufacturing Companies for Essential Amino Acids - L-Proline CAS NO 147-85-3 for Pharma Grade(USPEP) – Honray
Manufacturing Companies for Essential Amino Acids - L-Proline CAS NO 147-85-3 for Pharma Grade(USPEP) – Honray Detail:
Usage:
L-Proline is one of important amino acids which form protein in human boby. It is the essential material of amino acid transfusion. And it is also the main intermediate for compounding Captopril, Enalapril and other antihypertensive drugs. It has been widely used in food, medicine and other industries. It is made by fermentation of corn. So it is more safer.
Used as food additives. As special flavor agent, It can produce special aromatic substances when it is heated with sugar together.
L-Proline is one of important amino acids which form protein in human boby. It is the essential material of amino acid transfusion. And it is also the main intermediate for compounding Captopril, Enalapril and other antihypertensive drugs. It has been widely used in food, medicine and other industries. It is made by fermentation of corn. So it is more safer.
1. Used as nutritional supplement.
2. Used as the materials of compound amino acid infusion. Used in malnutrition, protein deficiency disease, stomach disease, scald and protein supplement after surgery.
There are two kinds of L – proline. One is direct fermentation, using glucose and yellow short bacillus mutant or glutamic acid bacillus wild strains, L – proline obtained with microbial fermentation; 2 it is chemical synthesis to glutamic acid as raw materials, and anhydrous ethanol under the sulfuric acid catalytic esterification, and add triethanolamine to amine sulfate free, glutamic acid – delta – ethyl ester. Reoccupy metal reductant glutamic acid potassium borohydride reduction – delta – ethyl ester, raw proline, finally the purification can be rough on the proline. Small craft take L – glutamic acid esterification said 147 g, into three bottle neck, add 1 L, anhydrous ethanol mix cool to 0 ℃, and then add 80 ml – H2SO4, at 0-5 ℃ stirring reaction 1 h, 1 h at room temperature to continue response, response all clear. Under 20 ℃ and triethylamine to pH 8-8.5, precipitation white crystalline, 1 h at room temperature and stirring, let stand for 5 ℃ cooling filtration, crystallization, washing with 95% ethanol, drained after vacuum drying, to glutamic acid ethyl – delta – about 141 g. Melting point of 178-180 ℃, the yield of 80% 83%. [alpha] 32 + 29.8 d (C = 1 g/ml 10% HCl). Reduction in three flask into glutamic acid ethyl – delta – 175 g, add distilled water, 875 ml, stir cool to 5 ℃, add in another KBH4 53.9 g, about 1 h adds, at room temperature and 1 h reaction, heat preservation 50 ℃ reaction 3 h. Cooled to 0 ℃, add 6 mol/L HCl to pH4, filtering the filtrate, quick rough L – proline aqueous solution. Ion exchange resin – alumina column chromatography separation and purification raw will L – proline aqueous solution, with 4 ml/min flow entering into the 732 – H + resin exchange column charge 10 ml acid resin (1 g). First rinse with distilled water to neutral, then use 1 mol/L ammonia elution, collection of L – containing proline eluent (thin layer chromatography with silica gel G control). Condensed the eluent decompression to dry, then use a small amount of water dissolves, it into neutral alumina column, again with 60% ethanol elution solution (or a thin layer chromatography with silica gel G control).
Specifications:
Item | AJI92 | EP8 | USP36 |
Assay | 99.0% ~ 101.0% | 98.5% ~ 101.0% | 98.5% ~ 101.5% |
PH Value | 5.9 ~ 6.9 | — | — |
Specific Rotation | -84.5° ~ -86.0° | -84.0° ~ -86.0° | -84.3° ~ -86.3° |
Transmittance | ≥98.0% | clear & colorless | — |
Chloride(as Cl) | ≤0.02% | ≤0.02% | ≤0.05% |
Ammonium(as NH4) | ≤0.02% | ≤0.02% | — |
Sulfate(as SO4) | ≤0.02% | ≤0.03% | ≤0.03% |
Iron(as Fe) | ≤10ppm | ≤10ppm | — |
Heavy metal(as Pb) | ≤10ppm | ≤10ppm | ≤15ppm |
Arsenic(as As) | ≤1ppm | — | — |
Other amino acids | conform | conform | conform |
Loss on drying | ≤0.30% | ≤0.50% | ≤0.40% |
Residue on ignition | ≤0.10% | ≤0.10% | ≤0.40% |
Ninhydrin-positive substances | — | conform | — |
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Sticking to the principle of "Super Quality, Satisfactory service" ,We are striving to be a good business partner of you for Manufacturing Companies for Essential Amino Acids - L-Proline CAS NO 147-85-3 for Pharma Grade(USPEP) – Honray , The product will supply to all over the world, such as: Nepal, Argentina, Uruguay, In order to carry out our goal of "customer first and mutual benefit" in the cooperation, we establish a specialist engineering team and a sales team to supply the best service to satisfy our customers' requirements. Welcome you to cooperate with us and join us. We have been your best choice.
By Maureen from Johannesburg - 2017.04.28 15:45
We have been cooperated with this company for many years, the company always ensure timely delivery ,good quality and correct number, we are good partners.
By Adela from Afghanistan - 2018.04.25 16:46